TRAINING SCHOOL IN RIGA
The use of non-invasive sampled matrixes in the comet assay as well as different types of white blood cells
Riga, January 28th to 31st 2019
Organiser:
Nikolajs Sjakste Venue: University of Latvia, House of Nature, Jelgavas Street 1, Riga, LV 1004, Latvia
Instructors:
Andrew Collins
Roger Godschalk
Sabine Langie
Stefano Bonassi
Massimo Moretti
Mirta Milić
Participants
Ana Siskova - Czech Republic
Aneta Gajowik - Poland
Anja Haveric - Bosnia and Herzegovina
Carina Ladeira - Portugal
Carla Russo - Italy
Elisabeth Elje - Norway
Ezgi Eylul Bankolgu - Germany
Fisnik Asllani - Kosovo
Goran Gajski - Croatia
Ibbo Willems - Belgium
Katarzyna Sikorska - Poland
Khaled Habas - United Kingdom
Lada Zivkovic - Serbia
Magdalena Czajka - Poland
Maria Sanchez Flores - Spain
Merve Bacanli - Turkey
Nursen Basaran - Turkey
Program:
Tuesday 29th January (9:30 - 17:30)
Theoretical introduction on non-invasive sampling in the comet assay and importance of different WBC types. Topics will include:
- Andrew Collins: 'The use of different blood cell preparations in the comet assay.'
- Roger Godschalk: 'Pros and cons of isolating white blood cell subtypes for Comet analyses'
- Maria Dusinska: 'Use of lens/eye cells to study DNA damage.'
- Massimo Moretti: 'Use of saliva in exposure assessment.'
- Sabine Langie: 'Use of saliva and buccal cells in human biomonitoring: a small case study'
- Mirta Milic: 'The use of buccal cells as a non-invasive material in genotoxic studies.'
- Stefano Bonassi: 'Data pooling in molecular epidemiology'. & 'Epidemiological and statistical issues of human biomonitoring studies using the comet assay'
Wednesday 30th January (9:00-18:00)
Hands-on training with various WBC fractions and lens cells
- Parallel groups will rotate to allow all to attend both trainings - Lysis over lunch - Demo on scoring
Thursday 31st January (9:00-18:00)
Hands-on training with buccal cells & saliva in the comet assay:
- Parallel groups will rotate to allow all to attend both trainings - Lysis over lunch - Demo on scoring
Friday 1st of February (9:00-12:00)
- Data analysis and interpretation
- Open discussion
- Take home messages
- Closing of the training
Lectures from the course:
1. Choice of the best biomarkers from the best tissue - Sabine Langie
2. The use of different blood cell preparations in comet assay - Andrew Collins
3. Pro's and con's of using isolated WBC in human biomonitoring studies - Roger Godschalk
4. Use of lens/eye cells to study DNA damage - Elisabeth Elje
5. Statistical and epidemiological issues with the comet assay
6. Buccal comet assay - Explanation. Mirta Milic
7. Hands-on training with saliva in the comet assay - Massimo Moretti
8. Tips & Tricks
Mini-symposium presentations:
